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:: Volume 29, Issue 2 (Summer 2011) ::
J Dent Sch GYEAR__, 29(2): 62-68 Back to browse issues page
Cytotoxicity comparison of nanosilver coated gutta-percha with Guttaflow and normal gutta-percha on L929 fibroblast with MTT assay
yazdan shantiaee Dr.1, Seyyed Omid Dianat Corresponding Author: Dr. *2, Hossein Mohammad Khani, Alireza Akbarzadeh Baghban Dr.3
1- Dept. of Endodontics
2- Dept. of Endodontics , dianat@dent.sbmu.ac.ir
3- Dept. of Biostatistics
Abstract:   (7123 Views)

Background and Aim: Gutta-percha has been the most widely used root canal filing material because of its well-known low toxicity. A new kind of gutta-percha with nanosilver coating has been synthesized by Iranian researchers and claimed to have antibacterial an antifungal properties. The aim of this study was to compare the cyotoxicity of nanosilver coated gutta-percha, GuttaFlow (GF), and normal gutta-percha (GP) on mouse fibroblast cell line L929.

Materials & Methods: In this in vitro investigation, cytotoxicity was evaluated by fibroblast cell culture and its direct contact with test materials. 30 prepared specimens of each material inserted in test tubes and cell survival fraction was estimated by MTT test after 1hour, 24 hour, and 1 week. Two way ANOVA, one way ANOVA and Tukey HSD were used for statistical analysis.

Results: After 1 hour, cytotoxicity of 3 test materials was significantly different. Nanosilver coated gutta-percha had the highest cytotoxicity and GuttaFlow the lowest one (P<0.01). After 24 hour, no significant differences were observed (P=0.37). After 1 week, GuttaFlow showed significantly higher cytotoxicity than normal gutta-percha and nanosilver gutta-percha (P<0.001). However, this difference was not significant between nanosilver and normal gutta-percha (P>0.05).

Conclusion: In nanosilver gutta-percha group, the most cytotoxicity was observed after 1 hour while it was decreased with time and became equal to normal gutta-percha after 24 hours and after 1 week, it reached the lowest level among 3 test materials.

Keywords: cytotoxicity, Gutta-Percha, GuttaFlow, Nanosilver Gutta-Percha, Fibroblast cell line L929
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Type of Study: Original Article | Subject: Special
Received: 2011/06/7
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Shantiaee Y, Dianat S O, Mohammad Khani H, Akbarzadeh Baghban A. Cytotoxicity comparison of nanosilver coated gutta-percha with Guttaflow and normal gutta-percha on L929 fibroblast with MTT assay . J Dent Sch. 2011; 29 (2) :62-68
URL: http://jds.sbmu.ac.ir/article-1-884-en.html

Volume 29, Issue 2 (Summer 2011) Back to browse issues page
مجله دانشکده دندانپزشکی دانشگاه علوم پزشکی شهید بهشتی Shahid Beheshti University Dental Journal
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