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:: Volume 21, Issue 3 (Autumn (Special Issue) 2003) ::
J Dent Sch GYEAR__, 21(3): 19-24 Back to browse issues page
Evaluation of telomere length in Oral Squamous Cell Carcinoma
Kaveh Oloomi Dr., Mahammad Amin Tavakkoli Dr., Mahammad Bayat Dr., Behnam Eslami Dr. * , Mahammad Moshref Dr., Farhat Majidfar Dr.
assistant Prof.
Abstract:   (5274 Views)

Background & Aim: Squamous cell carcinoma is the most common cancer in oral cavity with aggressive behavior. Many researches are done about pathogenesis and detection methods for this cancer. The purpose of this research is the evaluation of the length of telomere in Oral Squamous Cell Carcinoma (OSCC) and its probably diagnostic value.

Methods & Materials: We used the sensitive PCR technique based on primer extention method to determine the telomere length. The procedure steps were 1) DNA isolation 2) PCR assay 3) Electrophoresis for assessment of The PCR products 4) AgNO3 staining. At last length of the telomere was determined in comparison with 1 Kb marker.

Results: In 5 cases of OSCC the telomere length was less than 5 Kb and in the other 5 samples it was between 5-5.5 Kb, but in all normal tissue samples length of the telomere was more than 5.5 Kb (9.4-10.6 Kb). The findings showed a significant statistical difference between two groups (P<0.01). Also with the more severe dysplastic changes, higher telomere shortening was seen.

Conclusion: Telomere length is shortened in OSCC compare with normal tissue and can be a useful marker for diagnostic aims.

Keywords: telomere, Oral Squamous Cell Carcinoma, OSCC
     
Type of Study: Original Article | Subject: Special
Received: 2010/12/22
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Oloomi K, Amin Tavakkoli M, Bayat M, Eslami B, Moshref M, Majidfar F. Evaluation of telomere length in Oral Squamous Cell Carcinoma. J Dent Sch. 2003; 21 (3) :19-24
URL: http://jds.sbmu.ac.ir/article-1-853-en.html


Volume 21, Issue 3 (Autumn (Special Issue) 2003) Back to browse issues page
مجله دانشکده دندانپزشکی دانشگاه علوم پزشکی شهید بهشتی Shahid Beheshti University Dental Journal
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