[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 37, Issue 1 (winter 2019 2019) ::
J Dent Sch GYEAR__, 37(1): 1-5 Back to browse issues page
D-1 Gene Polymorphism in Salivary Gland Tumors
Jannan Ghapanchi1 , Behnam Samavatian2 , Hamid Ghaderi3, Bijan Khademi4, Elham Mahmoudi Maymand5, Elaheh Ainy * 6
1- Associate Prof., Dept. of Oral & Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
2- Co first Author, Dental Student, Student Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
3- Undergraduate Student, Student Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
4- Professor of ENT, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5- Genetic Expert, Shiraz Institute for Cancer Research, Shiraz University of medical Sciences, Shiraz, Iran.
6- fAssociate professor, Research Institute for Dental Sciences of Shahid Beheshti University of Medical Sciences, Tehran, Iran , ainy.elaheh@gmail.com
Abstract:   (238 Views)
Objectives This study aimed to assess PD-1gene polymorphism in salivary gland tumors in patients referred to Khalili Hospital in Shiraz.
Methods This case-control study evaluated 48 patients with salivary gland tumors and 100 age- and sex-matched healthy controls. First, 5cc blood samples were obtained from patients and transferred to vials containing anti-coagulated EDTA. DNA was extracted, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed on the samples. The PD-1 gene genotype was determined using the Fermentas kit. After 24 hours of incubation, all the samples were electrophoresed. The genotypes were reported based on the size of bands, and the chi-square test was applied. To compare the alleles, the Fisher’s Exact test was applied. The Yates correction was used to compare the genotype and genotypic alleles based on the tumor grade.
Results The mean age was 44.81±15.69 years in patients and 46.54± 13.86 years in controls. Statistical analysis did not show any significant difference in PD1 gene polymorphism between the two groups (P=0.098). No significant correlation was found between the genotype frequency and lymph node involvement (P=0.06), tumor genotype (P=0.12), side (right or left) (P=0.22), tumor location (P=0.27), and size or invasion of the tumor to the surrounding tissue (P=0.14). PD1.3 genotype frequency did not differ significantly between malignant and benign tumors (P=0.6).
Conclusion This study did not reveal any significant difference in genotype frequency of PD1.3 in the patient and control groups; however, further studies are needed with a larger sample size to obtain more accurate results
Keywords: Polymorphism, Genetic, Programmed Cell Death 1 Receptor, Salivary Gland Neoplasms
Full-Text [PDF 534 kb]   (58 Downloads)    
Type of Study: Original Article | Subject: Special
Received: 2019/10/7 | Accepted: 2019/10/7 | Published: 2019/10/7
 
 
 
 
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghapanchi J, Samavatian B, Ghaderi H, Khademi B, Mahmoudi Maymand E, Ainy E. D-1 Gene Polymorphism in Salivary Gland Tumors. J Dent Sch. 2019; 37 (1) :1-5
URL: http://jds.sbmu.ac.ir/article-1-1755-en.html


Volume 37, Issue 1 (winter 2019 2019) Back to browse issues page
مجله دانشکده دندانپزشکی دانشگاه علوم پزشکی شهید بهشتی Shahid Beheshti University Dental Journal
Persian site map - English site map - Created in 0.16 seconds with 32 queries by YEKTAWEB 3991