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:: Volume 36, Issue 1 (winter 2018) ::
J Dent Sch GYEAR__, 36(1): 18-22 Back to browse issues page
In Vitro Effect of Hydroalcoholic Extract of Aloe Vera and 0.2% Chlorhexidine Mouthwash on Streptococcus Sanguinis, Streptococcus Salivarius and Streptococcus Mutans
Mohadese Shakerian * 1, Mohamad Mehdi Yaghoti khorasani 2, Soheila Doostaki 3
1- Dept. of Operative Dentistry, Dental School, Mazandaran University of Medical Sciences, Sari, Iran , shakerianrostami@gmail.com
2- Dept. of Endodontics, Dental School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
3- Dept. of Periodontics, Dental School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Abstract:   (652 Views)
Objectives: This study aimed to assess the antimicrobial effects of aloe vera hydroalcoholic extract and 0.2% chlorhexidine (CHX) mouthwash on Streptococcus sanguinis (S. sanguinis), Streptococcus salivarius (S. salivarius) and Streptococcus mutans (S. mutans) in vitro.
Materials and Methods: Four concentrations (25%, 50%, 75% and 100%) of hydroalcoholic extract of aloe vera were prepared. S. sanguinis, S. salivarius and S. mutans were cultured on tryptic soy agar, and a single colony was removed from each microorganism and the opacity of pure microbial suspension was set at 0.5 McFarland standard concentration. Brain heart infusion broth culture medium, different concentrations of aloe vera extract, 0.2% CHX mouthwash, negative (distilled water) and positive (penicillin) controls and 0.5 McFarland bacterial suspension were added to the tubes and incubated at 37°C for 5, 10, and 15 minutes. Then, the samples were cultured on tryptic soy agar and incubated at 37°C for 48 hours. Number of colony forming units (CFUs) in the groups was compared with two-way repeated measures ANOVA. For paired comparisons, Tukey's multiple comparisons test was used (P˂0.05).
Results: The results showed significant effect of hydroalcoholic extract of aloe vera (25, 50, 75, and 100%) on the tested microorganisms but the mean CFUs following the use of 0.2% CHX was significantly less than that after using different concentrations aloe vera extract (P<0.001). Regardless of the type of the material, the number of CFUs decreased with increased exposure time.
Conclusion: Aloe vera can be used alone or with CHX mouthwash to eliminate cariogenic bacteria.
Keywords: Aloe vera, Anti-Bacterial Agents, Streptococcus sanguis, Streptococcus salivarius, Streptococcus mutans
Full-Text [PDF 459 kb]   (123 Downloads)    
Type of Study: Original Article | Subject: Special
Received: 2018/06/10 | Accepted: 2018/06/10 | Published: 2018/06/10
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Shakerian M, Yaghoti khorasani M M, Doostaki S. In Vitro Effect of Hydroalcoholic Extract of Aloe Vera and 0.2% Chlorhexidine Mouthwash on Streptococcus Sanguinis, Streptococcus Salivarius and Streptococcus Mutans. J Dent Sch. 2018; 36 (1) :18-22
URL: http://jds.sbmu.ac.ir/article-1-1653-en.html


Volume 36, Issue 1 (winter 2018) Back to browse issues page
مجله دانشکده دندانپزشکی دانشگاه علوم پزشکی شهید بهشتی Shahid Beheshti University Dental Journal
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