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:: Volume 33, Issue 3 (Summer 2015) ::
J Dent Sch GYEAR__, 33(3): 196-204 Back to browse issues page
Cytotoxicity of Triple Antibiotic Paste and Calcium Hydroxide against Cultured Human Dental Pulp Fibroblasts
M. Hosseini Matin * , M. Zare Jahromi , M. Fesharaki , M. Ostad Sharif
Postgraduate Student, Dept. of Endodontics, School of Dentistry, Islamic Azad University, Khorasgan Branch, Isfahan, Iran. , marjan3488@gmail.com
Abstract:   (4662 Views)

  Objective: In necrotic immature teeth, intra canal medicaments such as triple antibiotic paste (TAP) and calcium hydroxide (CH) are used for root canal disinfection and regeneration treatment. However, the effect of these medicaments on dental pulp fibroblasts has yet to be known. This study aimed to assess the cytotoxicity of CH and TAP against cultured human dental pulp fibroblasts (HDPFs) obtained from third molars.

  Methods: In this in vitro study, fibroblasts were obtained from the dental pulp of two third molars. Fibroblasts were exposed to 0.1, 1 and 10 mg/mL concentrations of TAP and CH. Six samples were prepared of each medicament and fibroblast viability was evaluated after 72 hours. Data were analyzed using one-way and two-way ANOVA (p<0.001). The percentage of cell viability was calculated and the cytotoxicity of the medicament was categorized as severe (30%), moderate (30-60%), mild (60-90%) and non-toxic (>90%).

  Results: In TAP samples, only the 10 mg/mL concentration had a significant difference with the control group in terms of the percentage of cell viability and showed moderate cytotoxicity. In CH samples, the 1 and 10 mg/mL concentrations showed significant differences with the control group and were severely cytotoxic.

  Conclusion: Reduction in cell viability of fibroblasts by increase in concentration was significantly greater in CH compared to TAP group. Thus, in regeneration treatments, these medicaments must be used in concentrations with adequate therapeutic and insignificant adverse effects on fibroblasts.

Keywords: Calcium hydroxide, Cytotoxicity, Dental pulp, Fibroblast, Triple antibiotic paste.
Full-Text [PDF 316 kb]   (805 Downloads)    
Type of Study: Original Article | Subject: Special
Received: 2015/08/31 | Accepted: 2015/08/31 | Published: 2015/08/31
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Hosseini Matin M, Zare Jahromi M, Fesharaki M, Ostad Sharif M. Cytotoxicity of Triple Antibiotic Paste and Calcium Hydroxide against Cultured Human Dental Pulp Fibroblasts. J Dent Sch. 2015; 33 (3) :196-204
URL: http://jds.sbmu.ac.ir/article-1-1426-en.html

Volume 33, Issue 3 (Summer 2015) Back to browse issues page
مجله دانشکده دندانپزشکی دانشگاه علوم پزشکی شهید بهشتی Shahid Beheshti University Dental Journal
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