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:: Volume 32, Issue 3 (Summer 2014) ::
J Dent Sch GYEAR__, 32(3): 145-150 Back to browse issues page
Comparison of Biocompatibility of Various Membranes with Fibroblasts
T. Foroutan *, M. Ghasemi , S. Farhang
Assistant Professor , foroutan@khu.ac.ir
Abstract:   (2394 Views)

  Objective: Different techniques have been suggested for the repair of bone defects at the injured sites. Use of biomembranes, or application of plasma rich in growth factor (PRGF) at the site of proliferation of osteoblasts are among the suggested techniques. The current study aimed to compare the biocompatibility of human periodontal ligament fibroblasts (hPLF) cultured on Hypro-Sorb F, Pericardium and Tutodent resorbable membranes coated with PRGF.

  Methods: This experimental study was conducted on four resorbable membranes namely Hypro-Sorb F, Pericardium, Tutodent and Vicryl. Fibroblast cells isolated from the periodontal ligament of premolar tooth were passaged three times and 105 cells/cm2 were cultured on membranes coated with PRGF. After 72 hours, the cells were evaluated in terms of biocompatibility and alkaline phosphatase (ALP) activity. Statistical analysis was carried out using one-way ANOVA.

  Results: PRGF increased cell adhesion and Tutodent membrane coated with PRGF showed the highest cell adhesion compared with Hypro-Sorb F and Pericardium membranes (p=0.005).

  Conclusion: PRGF increases cell viability and ALP activity of cells on biomembranes. PRGF treatment increases the adhesion of fibroblast cells to these membranes.

Keywords: Alkaline phosphatase, Fibroblast, MTT, PRGF
Full-Text [PDF 161 kb]   (515 Downloads)    
Type of Study: Original Article | Subject: Special
Received: 2014/08/4 | Accepted: 2014/08/4 | Published: 2014/08/4
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Foroutan T, Ghasemi M, Farhang S. Comparison of Biocompatibility of Various Membranes with Fibroblasts. J Dent Sch. 2014; 32 (3) :145-150
URL: http://jds.sbmu.ac.ir/article-1-1305-en.html
Volume 32, Issue 3 (Summer 2014) Back to browse issues page
مجله دانشکده دندانپزشکی دانشگاه علوم پزشکی شهید بهشتی Shahid Beheshti University Dental Journal
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