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:: Volume 30, Issue 3 (Fall 2012) ::
J Dent Sch GYEAR__, 30(3): 143-154 Back to browse issues page
Histological Evaluation of Regeneration in Rabbit Calvarial Bone Defects Using Demineralized Bone Matrix, Mesenchymal Stem Cells and Platelet Rich in Growth Factors
Hossein Behnia Dr., Ahad Khoshzaban Dr., Mehrnaz Zarinfar , Fatemeh Mashhadi Abbas Dr., Hassan Bahraminasab Dr., Arash Khojasteh Corresponding Author: Dr. *
assistant Professor Dept. of Oral & Maxillofacial Surgery , arashkhojasteh@yahoo.com
Abstract:   (5085 Views)

Objective: Regeneration of large bone defects is a major challenge in maxillofacial reconstruction. The present study evaluated the efficacy of simultaneous use of demineralized bone matrix (DBM), mesenchymal stem cells (MSCs) and platelet rich in growth factors (PRGF).

Methods: Stem cells were isolated from the femoral bone marrow of rabbits and their stem cell nature was confirmed. PRGF was prepared by drawing 5 cc of blood from the heart of rabbits and its centrifugation. A total of four 8mm defects were surgically created in rabbits calvaria using a trephine bur. Third passage cells were loaded on DBM and stored in an incubator for 24 hours. DBM, DBM+PRGF, DBM+MSCs and DBM+PRGF+MSCs were used in groups 1, 2, 3, and 4, respectively. At 6 and 12 weeks rabbits were sacrificed using vital perfusion. Histomorphometric analysis was performed on 5 µm sections stained with Hematoxylin and Eosin (H &E). Data were analyzed using SPSS version 14 software and ANOVA test.

Results: Histomorphometric analysis of the sections at 6 and 12 weeks post-operation demonstrated 74.6% and 20.31% osteogenesis in group 1, 14.35% and 28.44% in group 2, 17.75% and 31.33% in group 3 and 18.94% and 37.21% in group 4, respectively. Percentage of new bone formation was higher in group 4. However, this difference was not statistically significant (P>0.05).

Conclusion: It seems that simultaneous use of DBM, PRGF and MSCs results in increased bone regeneration in 8 mm rabbit calvarial bone defects when compared to the application of MSCs or PRGF alone.

Keywords: Bone regeneration, Platelet rich in growth factors, Mesenchymal stem cells, Demineralized bone matrix, Tissue engineering
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Type of Study: Original Article | Subject: Special
Received: 2012/10/29
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Behnia H, Khoshzaban A, Zarinfar M, Mashhadi Abbas F, bahraminasab H, Khojasteh A. Histological Evaluation of Regeneration in Rabbit Calvarial Bone Defects Using Demineralized Bone Matrix, Mesenchymal Stem Cells and Platelet Rich in Growth Factors. J Dent Sch. 2012; 30 (3) :143-154
URL: http://jds.sbmu.ac.ir/article-1-1026-en.html
Volume 30, Issue 3 (Fall 2012) Back to browse issues page
مجله دانشکده دندانپزشکی دانشگاه علوم پزشکی شهید بهشتی Shahid Beheshti University Dental Journal
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